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Invitrogen
F(ab')2-Donkey anti-Rabbit IgG (H+L) Secondary Antibody, PE, eBioscience™
FIGURE: 1 / 1
Rabbit IgG (H+L) Secondary Antibody (12-4739-81) in Flow
Mouse splenocytes were stained with Anti-Mouse CD39 Rabbit Polyclonal Antibody, followed with 1.0 µg of F (ab')2 Anti-Rabbit IgG PE and subsequently with Anti-Mouse CD4 FITC (Product # 11-0041-82) and Anti-Mouse CD25 APC (Product # 17-0251-82). The histogram (right panel) demonstrates staining of CD39 on CD4+CD25. cells (red line) and CD4+CD25+ cells (green line), as gated in the dot plot (left panel).
Product Details
12-4739-81
Applications
Tested Dilution
Flow Cytometry (Flow)
1 µg/test
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Donkey
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1210761
Target
IgG
Antibody Form
F(ab')2 Fragment
Product Specific Information
Description: The anti-rabbit IgG polyclonal antibody reacts with the heavy and light chains of rabbit IgG, with minimal cross reaction to bovine, guinea pig, Syrian hamster, horse, human, mouse, rat and sheep serum proteins. Reactions to non-immunoglobulin rabbit serum proteins have not been detected.
Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.
Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis to detect polyclonal rabbit antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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